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It was never fully revealed what the exact cause for the creation of the infection was other than it originating from Joker's mutated blood. The Joker was mildly annoyed by this failure, remarking, "Note to self: Need stronger test subjects." He then activated the transport cell which had previously housed the Titan Henchman to take him into Extreme Isolation.
Noting that all the formula needed was a specific enzyme that could be found in the blood of Ra's al Ghul (due to his constant usage of the Lazarus Pit), Batman managed to locate the League of Assassins' stronghold underneath Arkham City in Wonder City, and obtained a sample of Ra's blood. Returning the sample to Freeze, Harley Quinn managed to steal the developed cure as Freeze was attempting to use it as a bargaining chip to force Batman to find his wife, Nora. Joker: Because now there's a teeny little bit of me in you too, Bats. Oh, c'mon. Don't tell me it's not what you always wanted. Look, we're running out of time, I need your help. I nearly had a cure; It was so close and then it was taken form me.
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Titan Joker is a giant monstrous version of the Joker and the final boss in Batman: Arkham Asylum. Titan Joker is about as tall as Killer Croc. He's an incredibly muscular monster, a lot of his bones such as a large amount of his ribs on both sides as well as his fingernails are sharpen and revealed, and his hair is now a mohawk. After his encounter with the TITAN-infused thug, Bane launched a crusade against the existence of the TITAN drug, and devoted himself to the eradication of the compound. However, Bane soon decided that it was best to use the TITAN to enhance his own strength instead. Realizing that he couldn't do it alone, Bane signaled Batman with a flare and lured him into the abandoned Krank Co. Toy Factory at Amusement Mile in the flooded section of Arkham City. There, Bane alerted Batman to the twelve TITAN containers remaining within Arkham City. Hank & Dove”, the third episode of this season brought the first major casualty in Red Hood’s war on his ex-family. In that episode, Jason’s plot to destroy the team revolved around murder, literally and thematically. After strapping a WayneTech explosive to Hank Hall’s chest, Jason Todd attempted to corrupt Dove with the promise of her lover’s safe release. Red Hood promised Dove that if she shot and killed him, Hank’s bomb would deactivate.
In Asylum, when the player go to visiting center, Joker would said a joke that about how two inmates in an asylum escape (which were the last joke that he told Batman in the ending), however, he will say: 'Oh hell, you've heard that before, haven't you?', which a reference of the ending. GREETINGS, GOTHAM, JOKER HERE. ARKHAM IS MINE. SOON I'LL UNLEASH MADNESS UNTOLD ONTO THE STREETS OF GOTHAM. BUT FIRST, DIRECT YOUR EYES TO THE ROOFTOPS AND WITNESS THE FINAL DESTRUCTION OF YOUR DEAR DARK KNIGHT! Hello! Anyone in there? No sense of humour. Oh, the strong and silent type, eh? Think you're safe behind that mask? Give me 20 minutes and a can opener and I'll have you whimpering like a schoolgirl. You might like it. Additionally, the Joker was still suffering from the side-effects of his TITAN overdose, which mixed with the toxins in his blood and mutated into a extremely toxic poison with an estimated six months to live. Joker was planning to use the TITAN again after he had a doctor, which he promptly sent into the incinerator, isolate the TITAN from his blood. In addition, the Joker had some of the TITAN formula that was isolated from his blood injected into one of his henchmen, and then sent him after Bane. In his final moments, Joker gloated over Batman, showing arrogance, overconfidence, and remorseless to his very core. He boasts that in the end, he would always save him, ignoring that the Joker ended up destroying his own cure. Despite dying, he was able to find humor in his own death and ultimately got the last laugh on Batman.It really is worth spending some time trying out some of the different settings. It's one of the reasons that you'd buy an expensive chair – tuning it to suit your build and frame might take some time, but the end result is well worth it. Joker: Think of it as running gag...(stabs Batman with a knife which causes the cure to drop to the ground and shatter) NOOOO!!!! Are you HAPPY now!?? In Arkham Knight, he wears a brighter purple suit with red covering most of his face, in the Family Matters DLC he occasionally wears a top hat. I've really got to hand it to Scarecrow. I've never been a fan of his concoctions up to now, but this batch, it's intoxicating. It really brings out the me in you. Another user then asked "I bet you're the closest, but how would this particular strain (which I assume is a result of the Titan Overdose Illness), transfer Joker's memories? Would the other titan infected goons pass on their memories if they transfused their blood? Is it just a plothole I'm reading too far into?". u/2Scribble replied :
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